Isolation: The action of isolating; the fact or condition of being isolated or standing alone; separation from other things or persons; solitariness.
– Oxford English Dictionary
The controversy over whether the SARS-CoV-2 virus has ever been isolated or purified continues. However, using the above definition, common sense, the laws of logic and the dictates of science, any unbiased person must come to the conclusion that the SARS-CoV-2 virus has never been isolated or purified. As a result, no confirmation of the virus’ existence can be found. The logical, common sense, and scientific consequences of this fact are:
- the structure and composition of something not shown to exist can’t be known, including the presence, structure, and function of any hypothetical spike or other proteins;
- the genetic sequence of something that has never been found can’t be known;
- “variants” of something that hasn’t been shown to exist can’t be known;
- it’s impossible to demonstrate that SARS-CoV-2 causes a disease called Covid-19.
In as concise terms as possible, here’s the proper way to isolate, characterize and demonstrate a new virus. First, one takes samples (blood, sputum, secretions) from many people (e.g. 500) with symptoms which are unique and specific enough to characterize an illness. Without mixing these samples with ANY tissue or products that also contain genetic material, the virologist macerates, filters and ultracentrifuges i.e. purifies the specimen. This common virology technique, done for decades to isolate bacteriophages1 and so-called giant viruses in every virology lab, then allows the virologist to demonstrate with electron microscopy thousands of identically sized and shaped particles. These particles are the isolated and purified virus.
These identical particles are then checked for uniformity by physical and/or microscopic techniques. Once the purity is determined, the particles may be further characterized. This would include examining the structure, morphology, and chemical composition of the particles. Next, their genetic makeup is characterized by extracting the genetic material directly from the purified particles and using genetic-sequencing techniques, such as Sanger sequencing, that have also been around for decades. Then one does an analysis to confirm that these uniform particles are exogenous (outside) in origin as a virus is conceptualized to be, and not the normal breakdown products of dead and dying tissues.2 (As of May 2020, we know that virologists have no way to determine whether the particles they’re seeing are viruses or just normal break-down products of dead and dying tissues.)3
Read more: Statement on Virus Isolation (SOVI)